5th Annual WML Academic Symposium

Poster Presentation
Time: 4:00-5:30
Presenter: Vidhi Manubhai Desai
Title: Diethylstilbestrol-Loaded Bovine Serum Albumin Nanoparticles: Optimization, Characterization, and Stability Studies
Developed under the guidance of Dr. Qinfeng (Sarah) Liu, Pharmaceutical Sciences

Particulate systems like nanoparticles have been increasingly used for the delivery of therapeutic and diagnostic agents. Nanoparticles are used in vivo to protect the drug entity in the systemic circulation and to deliver the drug at a controlled and sustained rate to the site of action. Bovine serum albumin (BSA) will be used in the formulation of nanoparticles for diethylstilbestrol (DES) drug. Diethylstilbestrol is poorly water soluble and poorly permeable to biological membrane so it is a BCS class IV. Firstly, the optimization of preparing diethylstilbestrol-loaded BSA nanoparticles will be accomplished using response surface methodology (RSM) with three factors at five levels and a total of 45 runs. BSA concentration, BSA to drug ratio, Aqueous to organic volume ratio will be selected as independent variables and particle size will be selected as response variables. DES-loaded BSA nanoparticles size range will be investigated using Malvern zetasizer. Characteristic properties like percent entrapment efficiency, drug loading, and thermal studies using differential scanning calorimetry (DSC) will be investigated on the optimized nanoparticles of size range 50-150nm. Stability studies will be carried out for one week at 4oC and 25oC temperature and chemical stability will be studied at pH 7 and 8 and 0.1X and 10X PBS, pH 7.4 buffer for DES-loaded BSA nanoparticles. All characteristic properties will be compared with two controls: DES nanoparticles with no BSA and BSA nanoparticles with no drug. This research study will include various aspects of nanoparticle formulation, characterization and effect of stability on their characteristic property.